Stroke is the primary cause of serious long-term disability in the United States (1). ... anti-Kv2.1 polyclonal antibody (1:500 in 3% BSA. RRID AB_2040162, Alomone Labs, ... with TBST and incubated with the primary antibody anti-VAPA (RRID ... Induction of stable ERplasma-membrane junctions by Kv2.1 ...
2020-09-14
advances.sciencemag.org
A more stable, synthetic analog of carnosine, carnosinol, was recently ... while GADL1 acts on Asp, indicating that side-chain length is one determinant of ... Membranes were blocked with 3% BSA in TBST for 2 hours at room ...
2020-09-14
advances.sciencemag.org
When injected in mouse blastocysts, naive hPSCs generated a large amount of ... A transient inhibition of mTOR by rapamycin (10 M for 3 hours) also ... buffer, blocked with 5% milk in TBST (1 TBS + 0.1% Tween 20) for 60 min, ... for permeabilization, blocked in 3% BSA for 1 hour at room temperature, ...
2020-09-14
advances.sciencemag.org
3, E and F). A similar phenotype with altered spindle length and M I chromosome plate width was observed when the SAC was inactivated with ...
2020-09-14
advances.sciencemag.org
Therefore, we confirmed that AGP has a storage stability fixed to satisfy therapeutic needs. ... (B and C) Alga-gel reduced HIF-1 on high glucoseinduced HSF (n = 3). ... Skin flaps of about 1 cm (width) by 2 cm (length) were elevated on ... with 5% bovine serum albumin (BSA) blocking solution for 30 min.
2020-09-14
advances.sciencemag.org
The negative control bovine serum albumin (BSA) protein, which lacks ... Antibodies, diluted 1:1000 in 3% BSA or 5% milk in TBST, were added ...
2020-09-14
advances.sciencemag.org
Overexpression of Tcstv1 or Tcstv3 does not alter telomere stability. ... Average telomere length is shown as mean TFU SD. ... Nonspecific binding was blocked by incubation in 5% skim milk in TBST at room temperature for 2 h. ... blocking solution (3% goat serum plus 0.1% BSA in PBS) for 30 min at room ...
2020-09-14
www.nature.com
Administration of a long-acting GLP-1 receptor agonist or DPP4 inhibitor ... S2G), while obesity-induced hepatic caspase-3/7 activity and serum alanine ... A stable reading was obtained within 30 s, and upon reaching the current ... 0.2% FFA-free BSA, 2 mM glucose, and 2 mM pyruvate and then incubated ...
2020-09-14
advances.sciencemag.org
BSA-SSH was rapidly reduced using 50 nM TrxR1 and either 5 M Trx1 or 2 M TRP14 (Fig. ... membranes, demonstrated by the large amounts of proteins removed ... cellular context, we constructed stable TrxR1 and TRP14 knockdown ... Membranes were washed again three times in TBST buffer, and the ...
2020-09-14
advances.sciencemag.org
... in TBST with 5% (w/v) nonfat dry milk or 3% bovine serum albumin ... the impact of long-term effects of radiation on a cells ability to replicate. ... A NCI-H2228-Luc stable pool cell line was selected with puromycin (2 g/ml).
2020-09-14
advances.sciencemag.org
